Polymerase Chain Reaction (PCR) is a process where millions of copies of DNA are amplified from a single copy, or low copy number template. This reaction is used in all laboratories working with DNA and RNA.
Because of the high copy number generated during PCR, it is essential to prevent possible contamination of the PCR reaction.Reagents should be prepared in the reagent preparation area and transferred to the sample preparation area, through a pass box, or inside closed containers. After preparation of the final reaction mix, the tubes should be transferred to the amplification area, again through a pass box or in a closed container. The PCR amplification and results analysis takes place in this area.
To guarantee contaminant-free samples, it is essential to work in an environment where the air quality is controlled. This should form part of the equipment in the sample preparation area.
Construction Details - fabricated of industrial grade stainless steel grade SS-304 or PCRC body as desired. The inner portion painted with epoxy paint coating for extra-long life. The side panels made glass sheets are mounted on anodized aluminum frames.
Noise Level - Cabinets are designed to ensure minimum possible noise and vibration levels.
Filter Assembly
laminar air flows are fitted with fully washable synthetic pre-filter units and secondary high efficiency air filters made of mini pleated non-woven fabric. The efficiency of filters have a rating of better than 99.99% at DOP (cold) and 99.97% at DOP (Hot). Filter units have the capacity to hold all suspended particles of size greater than 0.3 micron.
Motor and Blower Assembly
laminar air flow units are provided with perfectly balanced (Static as well as dynamic) motor and blower motors bearing ISI mark. The rating of the assembly is 1/5 HP. high efficiency pumps which have life log lubricated bearings ensure a trouble free operation for a long time.
Ultra Violet Light
Optimal wattage ultra violet light is incorporated in the illumination panel of laminar air flow to take care of the sterilization of the existing air present in the enclosure, thus ensuring the high standard of cleanliness
