Soil Salinity And Its Measurement Soil Salinity Tester

July 2nd, 2014 by Acmas No comments »

Soil is the medium of growth of natural vegetation on earth’s surface. Various factors affect its composition such as climate, water level, biological properties and Salinity etc. The Salinity of soil refers to the amount of salts in the soil and it can be estimated by measuring the electrical conductivity (EC) of an extracted soil solution (EC increase with increase ion concentration). Salinity measurements are an important aspect of soil, crop and environmental protection, research and management.

Soil Salinity Tester measures the total amount of soluble salts in solution in a given soil sample. These tester based on electrical conductivity or total dissolved solids/ions measurements.

Applications of Soil Salinity Tester are:

  • Efficient monitoring of Soil Salinity.
  • Plant nutrient status and Fertigation.
  • Pollution and land reclamation.
  • Soil moisture mapping.

Two common ways for measuring Soil Salinity

  • Electrical Conductivity; EC meters measures how much electricity moves through a solution – the saltier the solution, the more the electricity moves through it, and higher the conductivity reading. EC for soil is measured in dS/m (deciSiemens/metre).
  • Total dissolved solids or total dissolved ions; measures how much salt is dissolved in soil solution. It is measured in units of mg/l (milligrams/litre) or ppm (parts per million). Higher readings mean more salt is present in the solution.

Effect of Soil Salinity on Plant Growth

Salinity can affect plant growth in several ways, directly and indirectly. High concentration of salt in soil can deteriorate the agricultural value of land.

Direct soil salinity damages

  • Decreased water uptake; High salts concentration results in high osmotic potential of the soil solution, so the plant has to use more energy to absorb water. Under extreme salinity conditions, plants may be unable to absorb water and will wilt, even when the surrounding soil is saturated.
  • Ion-specific toxicity
    When a plant absorbs water containing ions of harmful salts (e.g. Sodium, Chloride, excess of Boron etc.), visual symptoms might appear, such as stunted plant growth, small leaves, marginal necrosis of  leaves or fruit distortions.

 

Indirect soil salinity damages

  • Interference with uptake of essential nutrients
    An imbalance in the salts content may result in a harmful competition between elements. This condition is called “antagonism“, i.e. an excess of one ion limits the uptake of another ion.
  • Sodium effect on soil structure
    In saline soils, sodium replaces calcium and magnesium, which are adsorbed to the surface of clay particles in the soil. Thus, aggregation of soil particles is reduced, and the soil will tend to disperse. When wet, a sodic soil tends to seal, its permeability is dramatically reduced, and thus water infiltration capacity is reduced as well. When dry, a sodic soil becomes hard has the tendency to crack. This may result in damages to roots.

Factors affecting soil salinity

  • Irrigation water quality; the total amount of dissolved salts in the irrigation water, and their composition, influence the soil salinity. Therefore, various parameters, such as source water EC and its minerals content should be tested.
  • Fertilizers applied; The type and amount of fertilizers applied to soil, affect its salinity. Some fertilizers contain high levels of potentially harmful salts, such as potassium chloride or ammonium sulphate. Overuse and misuse of fertilizers leads to salinity buildup, and should be avoided.
  • Field’s characteristics;a poorly drained soil might reach salinity level that is harmful to the plants and to the whole crop. A soil that was not flushed after a previous growing cycle might contain high level of accumulated salts.

Seed Germinator As Tissue Culture Equipment

July 1st, 2014 by Acmas No comments »

seed germinator is Tissue/Cell Culture equipmentUsing Seed Germinators, varying atmospheric conditions can be created within the chambers without the need of going outside. Typically, these create an environment in which light, humidity and temperature are controlled to provide optimum conditions for the germination of seeds. They are made of double walled structure having two chambers, one inner and one outer. The main components of a Seed Germinator include;

  • A germinating cylinder
  • A temperate controller

Seed Germinators are designed in such a way that they are able to control the crucial environmental parameters.

Tissue/cell culture is a biological method of asexual propagation where a very small piece of tissue (shoot apex, leaf section, or even an individual cell) is excised (which is known as Explants) and placed in sterile /aseptic culture in a test tube/petri dish/tissue culture container containing a special culture media.
tissue/cell culture is used for rapid multiplication of an organism. A very specialized laboratory with sterile/aseptic conditions is required for tissue culture. All the procedure involved in tissue culture should be carried out in a aseptic conditions.

Steps involved in Tissue/cell Culture are

  • Explants ; excision of the plant parts.Place the explants in a tissue culture container/test tubes/petri dishes.
  • Multiplication ; Tissue grows and produce small plants.(Rapid multiplication by transfer of fresh culture).
  • Transplanting the plant into some kind of acclimation container.

Tissue culture methods are applied for obtaining the top seed germination rates and asexual propagation. In case of Plant tissue culture, the seed germination in vitro is one of the important steps, that requires optimal and controlled environmental conditions, and these conditions are provided by the Seed germinators.

Seed germinators have their applications in the following areas too;

  • Forestry research
  • Seed testing
  • Biological products
  • Plant tissue cultute

Seed & its Germination

July 1st, 2014 by Acmas No comments »

 

Seed is the outcome of sexual reproduction in flowering plants. It represents the beginning of a new generation. A Seed is defined as a fertilized mature ovule that possesses an embryonic plant, stored food material and a protective coat.

Factors affecting seed germination

Essential environmental factors for seed germination are;

  • Oxygen content; for germination, oxygen is needed in large quantities. The Seeds obtain this oxygen from the air contained in the soil. Seeds sown deeply in soil, fail to germinate because of lack of oxygen. Ploughing aerates the soil and helps in good germination. Respiratory rate of Dormant Seeds is very low so they require less amount of oxygen.
  • Temperature; Germination can take place over a wide range of temperature (5 – 40o C), but the optimum for most of the crops is between 25o C to 30o C
  • Moisture content or water; Water makes the Seed coat soft, causes it to rupture after swelling and start Germination. Water is also needed to bring in the dissolved oxygen for use by the growing embryo. In Dormant Seeds water content is 10-15% and is normally dehydrated, so the Dormant Seed have to absorb water to become active and for its germination.
  • Light; Light has varied effects on germinating Seeds of different plants. Some Seeds need light for Germination, while in some Seeds Germination is hindered by light (i.e. Short day plants and long day plants).

Seed Germinator

seed germinator is laboratory equipment which is used for the Germination of Seeds. Using Seed Germinators, varying atmospheric conditions can be created within the chambers without the need of going outside. Typically, these create an environment in which light, humidity and temperature are controlled to provide optimum conditions for the germination of seeds. They are made of double walled structure having two chambers, one inner and one outer. The main components of a Seed Germinator include;

  • A germinating cylinder
  • A temperate controller

Seed Germinators are designed in such a way that they are able to control the crucial environmental parameters.

Seed germinators can be of various types

  • For research purpose there are research Seed Germinators
  • There are economical germinators that are used as sprouters in different tissue culture industries and agro based industries

Apart from the usual germination applications, these Seed Germinator are also used for other testing application involving a plants, tissues, microorganism, electronic components etc.

Seed Germinators are also used in

  • Enzyme reaction studies
  • Tissue culture application
  • Growth observation studies
  • Fermentation analysis