UV Cabinet

August 4th, 2014 by Acmas No comments »

Ultraviolet light is, by definition, light with shorter wavelengths than the light which may be observed by the human eye. UV light is consists of three components

UVA, consisting of light with a wavelength between 400 and 320 nm;
UVB, consisting of wavelengths between 320 and 290 nm; and
UVC, with wavelengths between 290 and 200 nm,

For disinfection purposes, the optimal wavelengths reside within the UVC range. Ultraviolet light has been used in the Research Laboratory as an Effective Germicide and Virucide. UV light is a fairly efficient disinfectant for most vegetative organisms and viruses.

Mainly UV cabinets are used for Chromatographic analysis by Ultraviolet fluorescence. These Cabinets used for viewing Chromatogram combines both long and short wave Ultraviolet Lights and also White Light in a compact table-top unit. This self-contained unit is designed for easy viewing and making of Chromatograms and also for general Florescence Studies. The Cabinets have powerful UV Lights which serve to denature Nucleic Acids in 5 to 30 minutes making them unsuitable for Amplification and Contamination of PCR reactions. These lights are timer controlled and there are safety switches on the Cabinet Doors which automatically turn-off the UV lights when opened to prevent user exposure to UV light.

The Cabinets also act as an efficient shield against beta radioactive emissions and can therefore be safely used with Isotopes, such as ³²P. The Cabinet’s White Light provides excellent visibility when working inside them. And these Polypropylene Cabinet resists cracking and discoloration under extended exposure to UV light, as well as acids and other chemicals. Fan/filter module provides continuous wash of HEPA-filtered air to remove contaminants

Applications:

Long wave (Ultraviolet) component; Fluorescing spots on chromatograms can locate compounds like dye stuffs, vanillin’s flavones, alkaloids etc.
Short wave (Ultraviolet) component; Detects Compounds, such as Barbiturates, Purine and Pyrimidine derivatives, PHT-Amino Acids, DNP -Amino Acids and Steroids.
Denature Nucleic Acids in 5 to 30 minutes making them unsuitable for Amplification and Contamination of PCR reactions.
In detection of traces of minerals, study of old inspections.

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UV Trans-Illuminator

July 22nd, 2014 by Acmas No comments »

Ultraviolet (UV) Trans-illuminators are used to visualize fluorescent markers that are used in gel electrophoresis like agar gel electrophoresis for nucleic acids (DNA, RNA) and SDS-PAGE for proteins.

UV transilluminators are usually consist of a UV light source and associated electronics housed in a box with appropriate optical filters. For safety purpose it has a light transmitting cover with UV filter that transmits visible light to allow gels to be viewed. All UV trans-illuminator are major sources of UV radiations which can cause damage to skin and eyes of an individual. The use of UV trans-illuminator should be replaced whenever practicable by the use of blue light trans illuminator and appropriate dyes.

Risks from Radiation from UV trans-illuminator

These devices emit high level of UV radiations. UV radiation are mutagenic and cancerous, they can cause the mutation (UV radiations are responsible for the formation of dimers in DNA strands). Working unprotected for even a few minutes can cause injury, so it is required to use safety precautions while working with UV trans-illuminator. Effects of UV can occur within a short period (Photokeratitis and Erythema) or a long period after exposure (skin cancer).

Radiations from these UV trans-illuminators may have the potential effects on eyes (photokeratitis, photoconjunctivitis and cataracts) and
can cause skin erythema (sunburn like condition), elastosis ( photoaging and skin cancer).
Hands, arms, face and eyes are likely sites of injury.

Some individuals have greater than usual photosensitivity. It is possible to calculate acute threshold for acute effects and therefore set exposure limits, however it is not possible to do so for chronic effects, therefore there is no safe exposure level and exposure should be reduced as much as possible.

_{Safety Guidelines While Using the UV Trans-Illuminator}

_{UV Trans-illuminator is the source of UV radiations which can be harmful for a personnel doing the experiment so it is required to consider the safety precautions.}

_{Always avoid looking directly into the UV light. Only observe through the UV safety cover provided in the trans-illuminator if possible wear UV blocking spectacles during observation.}
_{Do avoid unnecessary exposure to eyes or skin. There is no safe exposure level to avoid skin cancer.}
_{While putting the gel in the UV trans-illuminator the personnel should wear the gloves as UV radiations are mutagenic.}
_{All the skin areas that are exposed must be covered including the wrist area upto the end of the lab coat sleeves.}
_{Appropriate shield (protective cover) should be used to prevent irradiation of the eyes and skin. An appropriate material for shield is Perspex.}

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Tissue Culture Microscope

July 15th, 2014 by Acmas No comments »

A microscope consists of a compound light microscope with high power objectives facing upward is known as tissue culture microscope. This is also known as inverted tissue culture microscope because the objective faces upward.

This inverted design of objective piece is required because the microscope is used to view the bottom of a Petri dish or flask since the culture is growing on the bottom of the dish. The higher the power of objective, the closer the objective needs to be to the specimen.

These microscopes are very expensive but are easy to operate. They provide useful information about the morphology and state of the cell by visualizing the cell with the help of these microscopes so that early signs of cell stress may be easily identified and appropriate action can be taken to prevent loss of cultures.

Cell/tissue removed from an organism, will continue to grow if they are supplied with the appropriate nutrients and conditions. When this process is carried in laboratory or in vitro the process is known as tissue or cell culture.

Plant Tissue Culture

Plant Tissue Culture refers to the culture of plant tissue/cells and organs such as root, shoot tips and leaves in artificial nutrient media aseptically.Growth and morphogenesis of tissue/cells in vitro are largely governed by composition of culture media.

Culture media used for the in vitro cultivation of plant cells are composed of following components

Essential elements, or minerals, supplied as a complex mixture of salts;
An organic supplement supplying vitamins and amino acids
A source of fixed carbon; usually supplied as the sugar sucrose
A gelling agent
Growth regulators (Auxins, cytokinines, Gibberellins, Abscisic acid and Ethylene)

Plant Tissue Culture is of many types such as callus culture, cell-suspension culture, protoplast culture, organ culture, meristem culture, root culture, embryo culture, haploid culture, anther culture and ovary culture.

Some important Applications of Plant Tissue Culture

Clonal Propagation; Multiplication of genetically identical copies of a cultivar by asexual reproduction is called clonal propagation. A population derived from a single individual by asexual reproduction constitutes a clone. Clonal propagation through tissue culture is known as Micropropagation.

Production of Virus free plants; infection can be eliminated by micropropagation of unaffected parts of plants. The basic method of production of virus free plant is culture of apical meristems.

Artificial seeds; somatic embryos may develop in vitro through embryogenesis. These can be preserved through the preparation of artificial seeds.

Production of secondarymetabolites; chemical molecules such as alkaloids, resins, tannins, latex, these chemicals are known as secondary metabolites. Their function is mainly associated with the defense against of pests and pathogens. By utilizing tissue culture technique these chemicals are produced in large scale.

Animal Tissue Culture

Cells in animals exist in an organized tissue matrix which require for their controlled growth and differentiation. These cells from intact organisms may be isolated, maintained and grown in vitro in culture media aseptically containing suitable mixtures of nutrients and growth factors, this process is called Animal Tissue Culture.

Some major applications of Animal Tissue Culture

Model System; animal cell culture provides a good model system for studying

Basic cell biology and biochemistry
Effects of drugs on cells
Interactions between diseases causing agents and cells
Nutritional studies

Cancer Research; since both normal and cancer cell can be grown in culture so there differences can easily be studies

Virology; replication of virus in cell culture for the production of vaccines.

Toxicity testing; cultured cells are widely used alone or in conjuction with animal tests to study the effects of new drugs, cosmetics and chemicals on survival and growth of cell.

Applications areas of Tissue Culture Microscope

Cellular biology
Molecular biology
Biology classroom equipment
Microbiological study
Environmental research
Medical diagnostics
Medical research