Archive for May, 2014

Dry Heat Sterilization-Ovens

May 2nd, 2014

Sterilization is the process of complete removal/destruction of the entire viable microorganism or Sterilization can be defined as any process that effectively kills or eliminates transmissible agents (such as fungi, bacteria, viruses and prions) from a surface, equipment, foods, medications, or biological culture medium.

Various methods used for sterilization are Heat (Dry and Moist), Radiation, chemical compounds and gases etc.

  • Moist heat sterilization is the most efficient Biocidal Agent. In the pharmaceutical industry it is used for Surgical dressings, Sheets, Surgical and diagnostic equipment, Containers, Closures, Aqueous injections, Ophthalmic preparations and Irrigation fluids etc.
  • Dry heat sterilization can only be used for thermo stable, moisture sensitive or moisture impermeable pharmaceutical and medicinal. These include products like; Dry powdered drugs, Suspensions of drug in non aqueous solvents, Oils, fats waxes, soft hard paraffin silicone, Oily injections, implants, ophthalmic ointments and ointment bases etc.
  • Gaseous sterilization is used for sterilizing thermo labile substances like; hormones, proteins, various heat sensitive drugs etc.
  • U.V light is perhaps the most lethal component in ordinary sunlight used in sanitation of garments or utensils.
  • Gamma-rays from Cobalt 60 are used to sterilize antibiotic, hormones, sutures, plastics and catheters etc.

Dry Heat Sterilizers

Hot air oven is one of the dry heat sterilizers. These sterilizers use dry heat for killing microorganism and are better than the steam sterilizers in the sense that the process does not corrode the machines. This is also used to sterilize liquids with low moisture content and also for treatment of medical powders. The process completely destructs all microorganisms and does not cause any environmental pollution or inconvenience to humans. Usually made up of stainless steel, the shape and position of heating elements in the chamber ensure uniform temperature distribution. The entire process includes drying, heating, sterilizing and cooling phase.

The benefit of dry heat includes good penetrability and non-corrosive nature which makes it applicable for sterilizing glass wares and metal surgical instruments. It is also used for sterilizing non-aqueous thermostable liquids and thermostable powders. Dry heat destroys bacterial endotoxins (or pyrogens) which are difficult to eliminate by other means and this property makes it applicable for sterilizing glass bottles which are to be filled aseptically.

Dry heat kills the organisms by destructive oxidation of essential cell constituents. Killing of most resistant spores by dry heat requires a temperature of about 160 degree C for 60 minutes.

Maintenance of Dry Heat Ovens

Maintenance of dry heat oven is an integral part of every dry heat sterilization procedure it can be achieved by

  • Keeping the oven clean at all times
  • Temperature gauge should be check on regular basis. This can be done by putting a thermometer in the oven and comparing the temperature reading with the one on the gauge.

Application of Dry Heat Sterilizers

  • Sterilizing laboratory instruments
  • Sterilizing ampoules
  • Sterilizing injection bottles
  • Sterilizing pharmaceutical vessels
  • Sterilizing medical instruments

Dissolved Oxygen and Importance of its measurements

May 2nd, 2014

Dissolved oxygen (DO) is the physical distribution of oxygen in water (as the oxygen does not react with water at all). A water body seldom has more than 10 ppm dissolved in water. Concentrations above this level can be harmful to aquatic life; fish in water body containing excessive dissolved gases may suffer from “gas bubble disease”.

Dissolved oxygen concentrations below 3 ppm stress most warm water species of fish and concentrations below 2ppm will kill some species. Often fish that have been stressed by Dissolved Oxygen concentrations in the range of 2 or 3 ppm become susceptible to diseases. The main sources of Dissolved Oxygen in water are

  • Atmosphere; wave action and splashing allow more oxygen to be absorbed into the water.
  • Photosynthesis; in the presence of light aquatic plants including algae; produce oxygen as a by-product during photosynthesis.

Factors affecting Dissolved oxygen in water are

  • Water temperature; Cold water can hold more oxygen than warm water.
  • Salinity; fresh water can hold more oxygen than salt water
  • Atmosphere; the greater the atmospheric pressure the more the oxygen water will hold.

Dissolved Oxygen increases with the decrease in temperature, salinity and an increase in atmospheric pressure.

As it is well known that,

  • 5-6 ppm; sufficient for most species
  • <3 ppm; stressful to most aquatic species
  • <2 ppm; Fatal to most species

Because of its importance to fish survival, aqua culturists, or fish farmers and aquarists measure the Dissolved Oxygen as a primary indicator of their system’s ability to support healthy aquatic life.

A Dissolved Oxygen Meter is used to measure the amount of oxygen present in a unit volume of water; it indicates if the water is useful for a specific application like water treatment plants, sewage treatment works, river monitoring and fish farming. It is a measure advantage for the aqua culturists, or fish farmers and aquarists.

Types of dissolved oxygen meter are

  • Polarographic (Clark cell method) sensor oxygen meter; use an external voltage keeping the potential difference between the anode and cathode to less than 0.5 volts. The working electrode uses a noble metal (Pt), and the opposite electrode uses Ag.

    For the electrolyte, a potassium chloride solution is used, and for the membrane, a Teflon membrane is used. Voltage is applied between the two electrodes so that the threshold diffusion current for oxygen is generated there. The oxygen which has passed through the membrane is reduced with the working electrode. A reduction current in proportion to the Dissolved Oxygen is generated, and then the Dissolved Oxygen is measured.

  • Galvanic Sensor Oxygen Meter; does not use an external voltage and the difference between the anode and the cathode is greater than 0.5 volts. These are more stable and accurate when compared to the Polarographic oxygen meters. The working electrode uses a noble metal (Ag), and the opposite electrode uses a base metal (Pb).

    For the electrolyte, an alkaline solution is used, and for the membrane, a highly oxygen-permeable Teflon membrane is used. Oxygen which has passed through the membrane is reduced with the working electrode. A reduction current in proportion to the concentration of the Dissolved Oxygen is generated, and then the Dissolved Oxygen is measured.

  • Optical Fluorescence Meter; are extremely suitable for long-term measurements in ground water as it is not sensitive to contaminats or for that matter, aging.

Uses of Dissolved Oxygen Meter are;

  • Analysis of boiler feed water for industries
  • Waste water treatment plants
  • Pollution control in rivers and lakes
  • Ionic concentration measurement for pharmaceutical companies
  • Analysis of drinking water

DEHUMIDIFIER

May 1st, 2014

DEHUMIDIFIER

Dehumidification is the removal of water vapor from air of an area.

Methods of Dehumidification

Cooling; Refrigeration of the air below its dew point (temperature at which air must be cooled at constant pressure in order for it to become saturated with respect to a plane surface of water) is the most common method of dehumidification. This method is advantageous when the gas is comparatively warm, has a high moisture content, and when the outlet dew point desired is above 40°F.

Liquid Desiccant; Liquid desiccant conditioners (absorbers) contact the air with a liquid desiccant, such as lithium chloride or glycol solution . The water vapor pressure of the solution is a function of its temperature and its concentration. Higher concentrations and lower temperatures result in lower water vapor pressures.

Dehumidification equipment: DEHUMIDIFIER

A Dehumidifier uses refrigeration principles same as the way an air conditioner does, but it doesn’t cool the air—in fact, it warms it slightly. Warm humid air from the structure/place is drawn into the cold dehumidification coil of the dehumidifier. Then the temperature of the air is lowered below the dew point condition and water (humidity) is collected on the dehumidification coil.

During the dehumidification process heat produced as water vapor becomes a liquid. This latent heat is then collected by the refrigerant system along with the heat taken from the entering air stream (sensible heat). Along with the latent heat, the energy used to operate the refrigerating compressor is also collected as heat energy and added to the refrigerant. Latent heat and compressor heat is then transferred by the refrigerant system to the condenser (re-heat) coil of the dehumidifier where it is added back into the air stream.

Construction of Dehumidifier

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A dehumidifier is a box that contains

  • cold evaporator coils,
  • warm condenser coils,
  • a fan, and
  • a reservoir or drain to catch water

The fan blows moisture-laden room air over the cold coils, where water condenses and drips into the pan or drain. The drier air then blows across the warm coils and back into the room.

Although a dehumidifier does not cool the air, it makes it more comfortable because it decreases the humidity. But a dehumidifier does not function well under about 65 degrees F.

A dehumidifier’s fan must have electric power, the evaporator coils must contain a proper charge of refrigerant, and the controls must work for the dehumidifier to operate.

In the same way that a thermostat controls a heater or air conditioner, a dehumidifier is cycled off and on by a humidistat that measures a room’s humidity.

Types of Dehumidifiers

There are many types of Dehumidifiers; some of them are listed below, each of its own advantage and disadvantages.

Heat pump dehumidifiers extract water from the air by using a heat pump that is similar to an air conditioner pump. First, a fan pulls the indoor air across a very cold coil, which causes the moisture to condense. The droplets drip into a bucket inside the unit. The dry air is warmed by a second, heated coil and exhausted at room temperature. These dehumidifiers are considered among the most effective.

Chemical absorbent dehumidifiers work best in warm climates. They use a chemical drying agent, or desiccant, on a heat exchange wheel to absorb water molecules from the air. Damp air is sent outside via vents. Desiccant dehumidifiers use a lot of energy. They are more commonly used by industries than homeowners.

Dehumidifying ventilators have a sensor controller and exhaust fan. The sensor controller activates the unit when humidity reaches a specified level. Dehumidifying ventilators are less effective in muggy climates because they draw outside air into the house. These dehumidifiers often are used in basements. But they can depressurize a room and cause gas spillage. Make sure gas furnaces are properly vented.

Portable dehumidifiers are the kind that you usually see in the home improvement aisle; they’re often plastic, relatively cheap and very lightweight. They’re designed to be most effective in smaller spaces like a bedroom or kitchen.

Restoration humidifiers are heavy-duty machines that can withstand harsh conditions — they’re usually used to repair heavy water damage caused by hurricanes or other natural disasters.

Application of Dehumidifier

Some commercial application of dehumidifier are:

• It lowers the relative humidity to facilitate manufacturing and handling of hygroscopic materials
• It lowers the dew point to prevent condensation on products manufactured in low-temperature processes
• It provides protective atmospheres for the heat treatment of metals
• It controls humidity in warehouses and caves used for storage
• It preservers ships, aircraft, and industrial equipment that would otherwise deteriorate.
• It maintains a dry atmosphere in a closed space or container, such as the cargo hold of a ship or numerous static applications
• It eliminates condensation and subsequent corrosion
• Drying air to speed the drying of heat-sensitive products, such as candy, seeds, and photographic film.
• Drying natural gas.
• Drying gases that are to be liquefied.
• Drying instrument air and plant air .
• Drying process and industrial gases .
• Dehydration of liquids .
• Frost-free cooling for low-temperature process areas such as  brewery fermenting, aging, filtering, and storage cellars; blast  freezers; and refrigerated warehouses.
• Frost-free dehumidification for processes that require air at a subfreezing dew point humidity.

Preventing the mold growth and allergies using Dehumidifier

Dehumidifiers can help mitigate the effects of common allergies to dust mites, fungus and mold; if the air at a place is excessively moist, it can encourage the growth of these allergens.Mold only requires a bit of moisture to grow, and it can set up shop at a place as soon as one of its airborne spores finds a hospitably damp surface. A mold problem can cause serious illness. And once it shows up, mold is a pain to eradicate and can permanently stain or damage whatever it’s decided to live on. The easiest strategy is to just keep it from showing up at all.

Safety Notes

1 .Do not connect unit to any AC electrical socket that is damaged.

2 .Ensure filter and removable water tank are correctly installed before turning on unit.

3. Always turn off the dehumidifier at the control panel when not in use and before unplugging.

4. Do not use it in the following locations:

i) Next to a source of heat

ii) an area where oil and water is likely to splash

iii) an area exposed to direct sunlight

iv) near a bath,shower or swimming pool( where the unit can get wet)

v) in a green house

5. Never insert your finger or other foreign objects into grills or openings.