Archive for May, 2014

Fruit Penetrometer

May 10th, 2014

Fruit hardness is the universally accepted measure of fruit ripeness. The fruit Penetrometer accurately measures fruit hardness by measuring the force required to push a plunger tip (of a certain size) into fruit and vegetables.

The GY series of Penetrometers are ideal for testing a wide range of fruits and vegetables. The force measurement provides the necessary information for growers to determine the best picking time, and to monitor fruit ripening and softening during storage.

Naturally, different fruits and vegetables will vary: varieties, geographical location and climate will all affect the appropriate firmness for picking a particular fruit. Recommended measurements are used as a guide, the growers using their experience and expertise to establish the precise firmness value applicable to their particular variety and environment.

The importance of measuring the firmness of fruit for testing maturity.
To talk about the maturity of fruit, its important to distinguish between physical maturity and commercial maturity. Physiological maturity can only transpire while the fruit is still on the plant and is the last moment when the fruit can be picked to be sold. Commercial maturity occurs later, and indicates the best time to consume the fruit, when its colour, flavour and texture are ideal for the consumer. In the case of the climatic fruit, the commercial maturity of the fruit can occur sometime after it has been picked.

While fruit is maturing, important changes occur inside, such as changes in colour, form and texture. It’s important to have objective values available with those that evaluate the changes in the fruit as it matures, to allow for decisions to be made as to when the fruit should be picked and processed (to determine the quality of fruit from the moment it becomes a product to its final consumption).

The firmness of the fruit, measured by the penetrometer, one of the most objective parameters in relation to the fruits maturity and as such is one of the most common techniques used in testing. Other methods are to determine the colour or the content of soluables using a refractometer (this technique is proportional to measuring total sugar and thus the sweetness of the fruit). If you are looking for refractometers to measure the sugar content of fruit, you can see them at this link.

Generally, the firmness or hardness of fruit measured by a penetrometer decreases as the maturity process occurs. Of course, you have to take into account that the firmness of the same fruit can vary, in general terms such as fruit variety or the region where it is cultivated, or due to its size or the temperature of the fruit when it is being measured by the penetrometer (the higher the temperature, the less firm the fruit is).

Penetrometers are used by fruit growers worldwide to help determine the harvest times for plums, navel oranges, nectarines, kiwifruit, peaches, and other varieties or stone or pome fruit. This penetrometer is the standard used by fruit growers for decades.

The plunger of the unit is pressed against the fruit and measurements of the rupture pressure can be seen on the gauge. Different varieties will have different rupture points. Can be used hand held or can be mounted on a drill press for additional accuracy.

Each unit comes with appropriate tips, a foam lined carrying case, a protective splash plate, a fruit peeler, a manual and recommended pressure test readings for specific fruits are including with each penetrometer. All units come with a one year “unconditional warranty.”

Vegetable hardness is a very important index in the quality control process. The main advantage of this instrument is that it is non-destructive. This means it has these advantages:

  • The maturation level of fruit can be monitored throughout the plant’s entire growth cycle;
  • Measurements can be carried out on the products where the classic fruit pressure tester (a destructive instrument) do not give acceptable results.

The instrument is very simple to use: once taken the instrument, place the plunger against the fruit and progressively press until the fruit is completely against the grey basement of the plunger.

For a more meaningful control it is necessary to take various samples of fruit. Two measurements must be carried out on each piece of fruit – on the opposite side of the fruit.

The determined value, average of the two measurements, is expressed in Shore degrees, range of reference for the hardness of a material.

Food Industry and Clean Benches (Laminar Air Flow)

May 8th, 2014

Main aim of all the food industries is to insure the safety of food that involves the careful control of process from the farm gate to the consumer. Safety includes control of both chemical and microbiological characteristics of the products. Most processing places emphasis on microbial control and often has its objective, the elimination of organisms or prevention of their growth.

Some of the aims of the food processing industry are

  • To make food safe ( microbiologically and chemically)
  • To provide products of highest quality ( flavor, color, texture)
  • To convert/make food into that forms that are convenient ( ease of use)

Items/points that should be controlled in food industry are

  • Microorganisms
  • Enzymes
  • Chemical reactions ( safety from a chemical viewpoint generally relates to keeping undesirable chemicals such as pesticides, insecticides and antibiotics our of the food supply)
  • Physical structure
  • Water activity

Clean bench/Laminar Air Flow is one of the major processes that are aimed at prevention of growth of microorganism. Laminar Air Flow/Clean Bench provides product protection by ensuring that the work in the bench is exposed to only HEPA filtered air. These are recommended for work with non hazardous materials where clean, particle-free air quality is required. Main aim is to keep the food product sterile and free of contamination.

Laminar Air Flow/Clean bench has its own filtered air supply, and are equipped with HEPA filters. HEPA filter removes particulates such as microorganisms, but it does not remove vapors/gases.

  • HEPA filters are made up of Boron Silicate Microfibers formed into a flat sheet by a process similar to making papers.
  • Flat filter sheets are pleated to increase the overall surface area.
  • Pleats are separated by aluminum baffles which direct the air flow through the filter.

Depending on its quality a HEPA filter can trap from 9,997 to 9,999 of every 10,000 particulates of a diameter greater than and less than 0.3 micron.

In the Baker (food) industry, HEPA filters used are leak free and rated at 99.99% performance level. But the Laminar Air Flow/Clean Bench does not provide protection to personnel or to the ambient environment and even it is not designed to contain aerosols generated by procedure; the user is exposed to these aerosols.

Major Equipments Involved in Microbiology

May 7th, 2014

Microbiology is the branch of biology that deals with the study of biochemical, physiological and genetic aspects of microorganism and how these creatures/microorganisms interact with each other. Microbiology is a vast area it includes bacteriology, environmental microbiology, food microbiology, microbial ecology and many more. This branch of biology involves;

  • Working in or creating aseptic conditions
  • Observations, identification and classification of microorganism
  • Detection of harmful microorganisms by performing tests on water, food and the environment
  • Studying human diseases caused by microorganism
  • Isolation and culturing of specific microorganisms under controlled conditions.
  • Isolating, analyzing and genetically manipulating nucleic acids, proteins and other substances produced by microorganisms
  • Isolating and genetically modifying microorganisms involved in breaking down pollutants
  • Developing genetically modified microbes for use in the production of genetically engineered biological products (proteins) or for gene transfer.

Microbiology helps scientists and physicians in the diagnosis, prevention and treatment of infection in animals and humans by investigating

  • How organisms cause disease and their role in disease processes
  • Factors contributing to the occurrence of diseases in a population
  • How epidemics can be controlled

A variety of specialized equipments are used in the microbiology, major equipments involved are:

Incubator shaker; the most common application of an Incubator Shaker in the Microbiology is for the growth of bacterial culture and other applications requiring both high temperatures and oscillation frequency.

An incubator Shaker uses an orbital agitation at variable speeds to affect the growth of cell cultures in addition to stable temperature conditions. Agitation speed and orbit affect both the aeration and the mixing of the cultures. Rate of cell growth increases with the increase in Oxygen transfer rate that depends on the aeration, in the other words the greater the aeration, greater is the Oxygen transfer rate and hence the rate of cell growth.

Autoclaves; An autoclave is a device that uses steam to sterilize equipment and other objects by subjecting them to high pressure saturated steam at 121 °C and 15 pounds of pressure per square inch depending for around 15–20 minutes on the size of the load and the contents.. This means that all bacteria, viruses, fungi, and spores are destroyed. Autoclaves work by allowing steam to enter and maintaining extremely high pressure for at least 15 minutes. Because damp heat is used, heat-labile products (such as some plastics) cannot be sterilized or they will melt.

Autoclave is commonly used for Moist Heat Sterilization. Moist heat is thought to kill microorganisms by causing denaturation or the coagulation of essential proteins. Autoclaving 121ºC/15 psi for 15 minutes exceeds the thermal death time for most organisms except some extraordinary spore formers .The time required to kill a known population of microorganisms in a specific suspension at a particular temperature is referred to as thermal death time (TDT). Temperature is inversely proportional to TDT. Processes conducted under high temperatures for short periods of time are preferred over lower temperatures for longer   times.
Lamina Air flow; Laminar Air Flow is an enclosed bench designed to prevent contaminations like biological particles (SPM) or any particle sensitive device. This closed cabinet is usually made up of stainless steel without any gap or joints where spores might collect.

Air is drawn through a High Efficiency Particulate Air (HEPA) filters and blown in a very smooth flow or streamline flow (when a fluid flows in a parallel layer with no disruption between the layers). There are two types of laminar air flow

  • Vertical
  • Horizontal

(Both types of hoods have continuous displacement of air that passes through a HEPA (high efficiency particle) filter that removes particulates from the air. In a vertical hood, the filtered air blows down from the top of the cabinet; in a horizontal hood, the filtered air blows out at the operator in a horizontal fashion.)

Centrifuge; A centrifuge uses centrifugal force to isolate suspended particles from their surrounding medium on either a batch or a continuous-flow basis. A centrifuge utilizes density difference between the particles/macromolecules and the medium in which these are dispersed and Dispersed systems are subjected to artificially induced gravitational fields.